熒光定量PCR服務要求

1. 請您提供新鮮的且盡量多的材料（各種材料的 RNA 提取量請參照“總 RNA 的提取”），或直接提供純化好的 總 RNA （大于 5 ug/ 樣品）；（ 各種材料的 DNA 提取量請參照“ D NA 的提取”），或直接提供純化好的 D NA （大于 5 ug/ 樣品 ) 。

2. 請通過 E-mail 提供已知的全長基因序列。

3. 請?zhí)峁┍M可能詳細的背景資料： DNA/ RNA 來源、豐度等

熒光定量PCR操作程序

1. 根據(jù)基因序列設計特異性的引物和熒光探針。

2. 提取 DNA/ RNA 。

3. Real Time PCR(熒光定量PCR)，進行實驗結(jié)果分析

4. 實驗完成后，提供完整的實驗報告（含軟件分析結(jié)果）及引物等實驗材料

熒光定量PCR收費標準
1、RNA提�。�10－20個樣品的RNA提取是：150元/個；20－30個樣品的RNA提取是：120 元/個；30個以上樣品的RNA提取是：100元/個；每份材料最少應提供：100mg/樣品
2、一個指標每個樣品為150元；二個指標每個樣品為240元；三指標每個樣品為300元，三個指標以上每個樣品為80元/反應。
3、引物合成為每個堿基2.1元，探針標記為1200元/條，如果是用sybr green I(染料)做，則免去探針的費用，染料贈送。
4、內(nèi)參的GAPDH引物探針免費，標準曲線免費贈送。
5、不再收取任何的基礎費或者開機費。

(如果樣本或者指標數(shù)更多，再面議)，5000.00元以內(nèi)的服務費，一律100%預付；

上海啟發(fā)生物科技有限公司自2003年成立以來，始終致力面向生命科學領域，從事科研機構(gòu)及生產(chǎn)企業(yè)所需的各類生產(chǎn)原料、試劑、儀器、耗材等的專業(yè)進出口公司。自成立以來發(fā)展迅速，產(chǎn)品服務得到全國廣大生產(chǎn)企業(yè)、高校、科研院所、醫(yī)療系統(tǒng)及相關試劑公司的肯定，長期合作的客戶遍及全國各地。并擁有自營進出口權(quán)，為更好地服務廣大用戶，不斷降低生產(chǎn)成本并向我們的客戶提供更強大的產(chǎn)品服務，同時為了將啟發(fā)生物的質(zhì)量管理提升到國際水平，啟發(fā)生物正在申請ISO9001國際質(zhì)量認證并已進入后期試運行階段。我們愿竭盡所能，與廣大生命科學工作者攜手迎接屬于生命科學的21世紀，共同創(chuàng)造新世紀的生物技術革命。
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重點品牌
RNA 干擾　　　　 Genetools 、Chemicon 、 Santa Cruz
信號轉(zhuǎn)導研究　　　Genetools 、Chemicon 、CST 、Santa Cruz　
分子生物學　　　　Toyobo 、Promega 、Strategene、Bio-Rad
干細胞、細胞凋亡　Chemicon　、R&D 、Santa Cruz 、Biovision
細胞因子及檢測　　Chemicon 、R&D 、Peprotech 、Prospec　
抗體與標記抗體　　Santa Cruz 、 R&D 、 Abnova、Cayman　
細胞培養(yǎng)及藥篩　　Clark、Cisbio、Hyclone 、Gibco、Chemicon
PCR與定量PCR　　Bio-Rad 、 Stratagene 、Promega 、Toyobo　
蛋白質(zhì)研究　　　　Pierce 、 Pharmacia 、 Pannomics 、 Bio-Rad　

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