本公司提供可培養多種細胞并能在細胞內穩定或瞬時轉染特定的受體或蛋白，以及耐藥細胞株的構建。 

Protocol  - 無菌操作和細胞培養操作規范

Aim
To ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross contamination with other cell lines.

Materials

• Chloros / Presept solution (2.5g/l)
• 1% formaldehyde based disinfectant e.g.Virkon,Tegador
• 70% ethanol in water (Prod. No.R8382)

Equipment

• Personal protective equipment (sterile gloves, laboratory coat, safety visor)
• Microbiological safety cabinet at appropriate containment level

Procedure

1. Sanitize the cabinet using 70% ethanol before commencing work.
2. Sanitize gloves by washing them in 70% ethanol and allowing to air dry for 30 seconds before commencing work.
3. Put all materials and equipment into the cabinet prior to starting work after sanitizing the exterior surfaces with 70% ethanol.
4. Whilst working do not contaminate gloves by touching anything outside the cabinet (especially face and hair). If gloves become contaminated re-sanitize with 70% ethanol as above before proceeding.
5. Discard gloves after handling contaminated cultures and at the end of all cell culture procedures.
6. Equipment in the cabinet or that which will be taken into the cabinet during cell culture procedures (media bottles, pipette tip boxes, pipette aids) should be wiped with tissue soaked with 70% ethanol prior to use.
7. Movement within and immediately outside the cabinet must not be rapid. Slow movement will allow the air within the cabinet to circulate properly.
8. Speech, sneezing and coughing must be directed away from the cabinet so as not to disrupt the airflow.
9. After completing work disinfect all equipment and material before removing from the cabinet. Spray the work surfaces inside the cabinet with 70% ethanol and wipe dry with tissue. Dispose of tissue by autoclaving.
10. Cell culture discard in chloros (10,000) ppm must be kept in the cabinet for a minimum of two hours (preferably overnight) prior to discarding down the sink with copious amounts of water.
11. Periodically clean the cabinet surfaces with a disinfectant such as Presept,Tegador or Virkon or fumigate the cabinet according to the manufacturers instructions. However you must ensure that it is safe to fumigate your own laboratory environment due to the generation of gaseous formaldehyde, consult your on-site Health and Safety Advisor.

資料來源： www.sigmaaldrich.com

佰而林生物科技有限公司，是一家由歸國留學專家創辦的專注于生命科學領域的高科技企業。公司提供覆蓋分子生物學、細胞生物學、免疫學等領域的實驗室產品、生物技術服務以及專業生物統計分析等咨詢服務，致力于為客戶在生命領域探索創造有利條件。經過三年的高速發展，已經在全國重點城市都成立了辦事處，與當地科研院校保持著廣泛的合作。

佰而林提供覆蓋臨床醫學、分子生物學、細胞生物學、免疫學、病理學等生命科學領域的生物技術服務，包括熒光定量PCR、免疫印跡、ELISA、流式細胞術、免疫組化、動物造模等。公司技術人員均具備本科以上專業背景，為客戶提供強大的技術保障。

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